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KMID : 1041219990410030147
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Journal of Sericultural and Entomological Science
1999 Volume.41 No. 3 p.147 ~ p.153
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Molecular Cloning and Characterization of the Gene encoding β- N-acetylglucosaminidases Homologue from Bombyx mandarina
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구태원/Goo, Tae Won
황재삼/성규병/윤은영/방혜선/권오유/Hwang, Jae Sam/Sung, Gyoo Byung/Yun, Eun Young/Bang, Hae Sun/Kwon, O Yu
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Abstract
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Chitinolytic enzymes such as ß-N-acetylglucosaminidase are major hydrolases involved in insect molting. We have isolated, sequenced a cDNA encoding ß-N-acetylglucosaminidase from the silk-worm, Bombyx mandarina, and compared its sequence with genes encoding chitinolytic enzymes from other sources. The insert DNA in the clone is 3,284 nucleotides long with an open reading frame of 1,788 nucleotides that encodes a protein of 596 amino acids with a molecular weight of 68.2 kDa. There is a 3´-untranslated region composed with 1,479 nucleotides and are several potential polyadenylation signals. The predicted amino acid sequence apparently contains a leader peptide of 23 amino acids. A search of the amino acids sequence databases for sequences similarities to other ß-N-acetylglucosaminidases or P-N-acetylhexosaminidases. The highest similarity matched with the enzyme from B. mori, which has a sequence identity of 95%.. On the other hand, the identity between the B. mandarina enzyme and those from M. Sexta and human are 70% and 24%, respectively.
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